| 1. | The ha 1 gene was inserted into the bacterial plasmid pgex - 4t - 2 and the recombinant plasmids containing ha 1 gene were identified by restriction enzyme analysis and pcr mathod
结果表明同源性分别达到98和97 ，并且在ha1切割位点有多个碱性氨基酸的插入序列，证明其为强毒株。 |
| 2. | For the products of primary rt - pcr and nested - pcr are all encompassing the hyper - variable region of vp2 gene , so we can take a restriction enzyme analysis ( rea ) directly
第二，本研究的基础rt - pcr和nested - pcr所扩增的片段均是横跨ibdv的vvp2的，所以，可直接对pcr产物进行酶切分型研究。 |
| 3. | Proper multi - copy gene was selected and cloned into puc19 vector . restriction enzyme analysis and dna sequencing confirmed that 5 - copy gene was correctly inserted into the vector
选取合适拷贝数的串连重复基因，将其克隆至puc19载体，双酶切、 pcr扩增和dna测序证明串连重复基因构建成功且基因方向相同。 |
| 4. | We confirmed the correct construction by pcr and restriction enzyme analysis . in this research , hypocotyls were used as the explant and several factors affecting genetic transformation of carrot mediated by agrobacterium tumefaciens were studied
利用vp7基因和质粒pbi121上相同的单克隆位点，将vp7基因定向克隆到植物表达载体pbi121上，构建了pbi121vp7表达载体。 |
| 5. | Conclusion : by restriction enzyme secting , ligating , transforming , restriction enzyme analysis , and final dna sequencing , the pbd - i and pbd - ii gene were proved to be recombinated with the expression vector and the recombinated vector ppd - 1 and ppd2 were transformed successfully
结论：经过酶切、连结，构建成重组质粒ppd上、 ppd上，再经转化、抽提质粒及酶切分析，最后经dna测序证实， rticr扩增的pbd i 、 pbd 11基因与piflpdt ” xsi表达载体构建成功。 |
| 6. | After the pbd i and pbd ii gene ligated with the expression vector pinpoint ? a - 3 , the recombinated plasmid ppd - 1 and ppd - 2 was transformed into jm109 strains , 4 positive clones were screened by restriction enzyme analysis , dna sequencing showed that two out of 4 positive clones inserted sequence of the constructed plasmid , which was the same as that of pbd - i and pbd - ii gene respectively , and its reading frame was correct , thus its could be used to express fusion protein
将pbd - 、 pbd -基因与表达载体pinpoint ~ ( tm ) xa - 3连结后获得的重组质粒ppd - 1 、 ppd - 2转化于大肠杆菌jm109中。经抽提质粒、酶切分析及pcr扩增，分别筛选到4个阳性克隆，将其中二个阳性克隆由测序分析，证实1个含pbd i基因片断， 1个含pbd 11基因片断，且阅读框正确，可用于融合蛋白表达。 |
| 7. | Abstract : the polymorphism of angiotensinogen gene at position 174 was studied in 90 cases of essential hypertension patients and 109 controls by pcr , restriction enzyme analysis and electrophoresis methods . the results showed the distribution of genetypes in hypertension group was significantly different from that of controls group . this suggested there is a correlation between the variant of agt174 and hypertension
摘要本文采用pcr 、限制性酶切和电泳分型等方法,分别对90例原发性高血压患者和109例正常人血管紧张素原基因多态位点agt174进行了检测,结果表明,高血压组中三种基因型的分布与对照组显著不同,提高该位点变异与原发性高血压的发生相关。 |
| 8. | Acetylornithine deacetylase is the key enzyme of producting l - methionine . we mainly do research work on the construction of acetylornithine deacetylase gene - engineering strain and characteristic of proteinase . in order to get high expression deacetylase strain , we obtain the gene by pcr arge gene . the product ( 2800bp ) was cloned into puc19 plasmid and confirmed with blue / white dot screening > restriction enzyme analysis and pcr . then taking the nucleotide sequencing compared with the sequence at blast of u . s . a . we constructed a high expression of gene - engineering strain - pxj 128 which containing the arge gene on the high expressing system of pxji18 with activity of acetylornithine deacetylase above 20000u / g
为了获得高效表达的脱乙酰鸟氨酸酶工程菌株，在工程菌技术改造及其固定化研究做了进一步的研究和探讨。我们采用基因工程技术，通过pcr技术扩增出了酰化酶关键酶基因?脱乙酰鸟氨酸酶基因arge ，将其克隆到puc19载体中，经酶切鉴定、 pcr鉴定筛选出重组阳性质粒，并测序鉴定，通过美国blast程序进行了基因数据库相似性比较分析。 |
| 9. | Ii ) two fragments ( about 240bp and 130bp ) were amplified from human keratinocytes total rna by rt - pcr . the recombinant pm - hpabl and pm - hpabs plasmids were constructed by inserting 240bp and 130bp pcr products into pmd 18 - t vector , respectively . the recombinants were identified by restriction enzyme analysis and dna sequencing , iii ) two orfs ( > 100bp ) were found in the insert sequence of pm - hpabl
应用smartpcr试剂盒和简并引物从人皮肤角质形成细胞cdna中扩增到长分别为24obp和13obp左右的2种片段，将它们插入pmd18一t载体，用酶切法初步筛选阳性重组子pm . hrabl和pm一hrabs ，对阳性重组子进一步作测序鉴定。 |
